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Of course you can do this.

Firstly you have to check your Apache version. You should have 2.4+ version. I will show you command for my server on Ubuntu 14.4.

Secondly, turn on necessary apache modules:

a2enmod proxy
a2enmod proxy_http
a2enmod proxy_wstunnel

Open conf of your domain, in my case that was a path to file:

/etc/apache2/sites-available/myDomain.pl.conf

Next, append this code

<VirtualHost>

.
.
.

RewriteEngine on

RewriteCond %{QUERY_STRING} transport=polling
RewriteRule /(.*)$ http://localhost:3001/$1 [P]

ProxyRequests off
ProxyPass /socket.io ws://localhost:3001/socket.io
ProxyPassReverse /socket.io ws://localhost:3001/socket.io

ProxyPass        /socket.io http://localhost:3001/socket.io
ProxyPassReverse /socket.io http://localhost:3001/socket.io
</VirtualHost>

Finally restart your apache

service apache2 restart
Have fun!